Studies of Aldehyde Oxidase by Electron Paramagnetic Resonance Spectroscopy

Studies of aldehyde oxidase by electron paramagnetic resonance spectroscopy. II. Kinetic studies by rapid freezing.

Aldehyde oxidase from rabbit liver was studied by electron paramagnetic resonance (EPR) spectroscopy. Reduction by substrate elicited three types of signals characteristic of free radicals, molybdenum(V), and a non-heme iron complex, respectively. The properties of these signals are described, and the representation of each species in the corresponding signal is quantitatively assessed. Under t...

Studies by electron-paramagnetic-resonance spectroscopy of the molybdenum centre of aldehyde oxidase.

Molybdenum(V) e.p.r. spectra from reduced forms of aldehyde oxidase were obtained and compared with those from xanthine oxidase. Inhibited and Desulpho Inhibited signals from aldehyde oxidase were fully characterized, and parameters were obtained with the help of computer simulations. These differ slightly but significantly from the corresponding parameters for the xanthine oxidase signals. Rap...

Electron Paramagnetic Resonance Spectroscopy

Electron paramagnetic resonance studies on the cytochrome oxidase from yeast.

I M. D. KAMEN, R. G. BARTSCH, T. HORIO AND H. DE KLERK, in S. P. COLO\VICK AND N. O. tXAPLAN, Methods in Enzymology, Vol. VI, Academic Press, New York, I963, p. 391. 2 T. HORIO AND M. D. KAMEN, Biochim. Biophys. Acta, 48 (I96I) 266. 3 G. COHEN-BAZIRE, ~V. R. SISTROM AND R. Y. STANIER, J . Cellular Physiol., 49 (1957) 25. 4 R. G. BARTSCH, in H. (;EST AND A. SAN PIETRO, Bacterial Photosynthesis, ...

Demystifying EPR: A Rookie Guide to the Application of Electron Paramagnetic Resonance Spectroscopy on Biomolecules

Electron Paramagnetic Resonance (EPR) spectroscopy, also known as Electron Spin Resonance(ESR) especially among physicists, is a strong and versatile spectroscopic method forinvestigation of paramagnetic systems, i.e. systems like free radicals and most transition metalions, which have unpaired electrons. The sensitivity and selectivity of EPR are notable andintriguing as compared to other spec...